Yes. The 54. round ball is enough to take Bison with a clean kill.
The key to any sportsmenship, is to know your own personal limits and accuracy of range of your firearm as well as the anatomy of the creature you choose to hunt.
Please do not be tempted too up-grade too larger projectiles and "magunm" loads..A heart shot is a heart shot. Don't expect your animal too "Fall Over" like a north carolina deer,,it's 600-700 pounds!
There are NO wild bison game hunting rules/laws today in the US..Bison are taken in a "box hunt" type situations only,,usually done by Ranchers needing too "cull" the herd. These animals are driven to a smaller enclosed area than the larger selection of breeding stock..
Buffallo is a fine meat, lean and healthy,,,,,,,