The standard way to check cylinder alignment is with range rods, but I find that pointing the gun at the sun, with my back to the sun, and barrel pointing at my best eye, hammer cocked, CHAMBERS DOUBLE CHECKED FOR EMPTY, that the face of the cylinder is clearly visable on one side if alignment is bad enough to cause accuracy problems. It will be very visable if bad enough to cause leading problems with the loads you are using.
Your pressures with Universal are considerably higher than I was speaking of, as I was using H110, a far slower burning powder. If you bullets are soft, like air cooled ww or even softer, the bases may be spreading out into the forcing cone after leaving the cylinder throats, causing an extreme drag, which could easily cause the leading problem.
Tell me how hard your bullets are, or what alloy your using, and whether you water drop, heat treat or what, so I have an idea of what hardness you are using. Perhaps going to H110 or 296 will be your solution, but don't do it yet. I consider Universal the optimum revolver powder for a packing gun, as it gives less smoke and muzzle blast than any powder I've ever used. You should be able to get your speed without leading if your bullets are hard enough. 20 bhn is plenty.