You can come pretty close to getting your desired chamber size by knowing the density of black powder and the final volume of the chamber.
The density of black powder changes with grade and humidity, but if you use 250 grains per cubic inch you'll be on the safe side.
To calculate the chamber volume, square the radius, multiply by pi (3.14), and then multiply the chamber length (all in inches) to get the volume of a pure cylinder. Multiply that by the density, and you have your powder charge.
For example, a 1" x 2" long chamber would be (0.5" x 0.5") x 3.14 x 2" = 1.57 cubic inches.
1.57 c.i. x 250 gr./c.i. gives you 392.5 grains capacity.
This formula is for a perfectly cylindrical chamber. If you have a hemispherical bottom on your chamber, that reduces the effective length of your chamber by about 1/2 of a radius.
This should get you in the ballpark, but remember to weigh a full charge just to be sure (and leave a full caliber wall thickness).
Tom